12041677001

CDP-Star^®, ready-to-use

>98%, solution, suitable for dot blot, suitable for Northern blotting, suitable for Southern blotting

• UNSPSC 코드: 12352204

속성

• 설명: Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate
• Quality Level: 100
• 분석: >98%
• 양식: solution
• 포장: bottle of 2 × 50 mL ([0.25 mM])
• 제조업체/상표: Roche
• 기술: Northern blotting: suitable; Southern blotting: suitable; dot blot: suitable
• 저장 온도: 2-8°C

설명

애플리케이션

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:

• Southern blot
• Northern blot
• Dot blot
• Colony and plaque hybridization
• Gel shift assays

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:

• Southern blot
• Northern blot
• Dot blot
• Colony and plaque hybridization
• Gel shift assays
• Chemiluminescent detection.

특징 및 장점

Contents
0.25 mM solution (0.124 mg/ml), ready-to-use, colorless solution

CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.

• Save time with a fast, ultra-sensitive substrate.
• Easily strip and reprobe membranes.
• Take multiple exposures of up to two days for each experiment.

Reuse the substrate by filtering and storing in sodium azide.

제조 메모

Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C

기타 정보

• First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
• No preincubation step required.
• Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
• Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
• Exposure times are shorter than with CSPD.
• Do not use nitrocellulose membranes.

Repeat exposures can be made up to two days after the addition of substrate.

For life science research only. Not for use in diagnostic procedures.

법적 정보

CDP-Star is a registered trademark of Tropix, Inc.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash