Name: IPTG
Brand: SIAL

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About This Item

실험식(Hill 표기법):

C₉H₁₈O₅S

CAS Number:

367-93-1

Molecular Weight:

238.30

Beilstein:

4631

EC Number:

206-703-0

MDL number:

MFCD00063273

UNSPSC 코드:

12352201

PubChem Substance ID:

24896093

NACRES:

NA.31

기술 서비스

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도움 문의

생물학적 소스

synthetic

Quality Level

200

설명

non-ionic

분석

≥99% (TLC)

양식

powder

기술

thin layer chromatography (TLC): suitable

불순물

≤0.1% Dioxane

색상

white to off-white

유용한 pH 범위

5-7 (50 g/L)

mp

105 °C ((221 °F))

solubility

water: 0.05 g/L, clear

응용 분야

cell analysis
life science and biopharma

저장 온도

2-8°C

SMILES string

CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

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일반 설명

Isopropyl β-D-Thiogalactopyranoside (IPTG) is a molecular biology reagent used to induce gene expression in bacterial systems. It mimics lactose, binding to the lac repressor protein, leading to gene activation. IPTG is crucial for studying gene regulation, protein expression, and recombinant protein production, providing valuable insights into cellular and molecular biology research.

애플리케이션

IPTG has been used:

in the induction of protein expression in Lactobacillus reuteri GroEL and mutants
in the induction of protein expression in Escherichia coli

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

생화학적/생리학적 작용

IPTG is a non-metabolizable analog of allolactose. It acts as an inducer that regulates the transcriptional activity of the lac promoter, a promoter that is used for the expression of the protein in E.coli.

기타 정보

Non-metabolizable galactose analog.

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재구성

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.