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43816

Sigma-Aldrich

DL-Dithiothreitol solution

BioUltra, Molecular Biology, ~1 M in H2O

동의어(들):

Cleland′s reagent, (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, (±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT

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About This Item

실험식(Hill 표기법):
C4H10O2S2
CAS Number:
Molecular Weight:
154.25
Beilstein:
1719757
MDL number:
UNSPSC 코드:
12352201
PubChem Substance ID:
NACRES:
NA.25
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도움 문의

Grade

Molecular Biology

Quality Level

제품 라인

BioUltra

분석

900-1100 mmol/L (Titration molarity)

양식

liquid

반응 적합성

reagent type: reductant

농도

~1 M in H2O

불순물

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH

2.7-5.0

density

1.04 g/mL at 20 °C

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유사한 품목 비교

전체 비교 보기

차이점 표시

1 of 4

이 품목
276839229997746355
Zinc chloride 99.999% trace metals basis

229997

Zinc chloride

Zinc chloride anhydrous, free-flowing, Redi-Dri™, ACS reagent, ≥97%

746355

Zinc chloride

form

liquid

form

liquid

form

crystals and lumps

form

powder, crystals or granules

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

density

1.07 g/mL at 25 °C

density

0.835 g/mL at 25 °C

density

-

density

-

reaction suitability

reagent type: catalyst
core: zinc

reaction suitability

-

reaction suitability

reagent type: catalyst
core: zinc

reaction suitability

reagent type: catalyst
core: zinc

greener alternative product characteristics

Catalysis
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

greener alternative product characteristics

-

greener alternative product characteristics

-

sustainability

Greener Alternative Product

sustainability

-

sustainability

-

sustainability

-

일반 설명

Dithiothreitol (DTT), a thiol group protectant,[1] is an excellent reagent for preventing the oxidation of SH groups in proteins. DTT solution is a potent and versatile disulfide-reducing agent.[2] It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange.

애플리케이션

DL-dithiothreitol solution has been used:

  • in the treatment of intestinal explants[3]
  • in homogenate preparation of gill cells for enzymatic assays[4]
  • in protein electrophoresis for western blotting[5]
  • in transglutaminase activity[6]
  • in sample buffer for SDS-PAGE[7]
  • in Western Blot, as a lysis buffer component for single-nucleus RNA-sequencing[8]
  • as a component of protein extraction buffer for cell lysis[8]

생화학적/생리학적 작용

DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms.[9] It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.[10]Dithiothreitol (DTT) is broadly utilized in synthetic peptide synthesis and biochemical arrangements of thiol proteins. It can also be used to study protein chemistry topics like enzyme activity and protein folding. DTT explicitly controls the thiol-disulfide exchange response to completely reduce the intra-or between sub-atomic disulfide bonds in biomolecules. Thiols and the cyclic disulfide of DTT are the products of this reaction.

특징 및 장점

  • BioUltra grade - 1M DTT solution, suitable for molecular biology
  • Free from DNases, RNases, phosphatases, and proteases

기타 정보

For additional information on our range of Biochemicals, please complete this form.

픽토그램

Corrosion

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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문서

Colloidal quantum dots (CQDs) are semiconducting crystals of only a few nanometers (ca. 2–12 nm) coated with ligand/surfactant molecules to help prevent agglomeration.

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