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About This Item

Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
Beilstein:
505995
EC Number:
MDL number:
UNSPSC 코드:
12352111
PubChem Substance ID:
NACRES:
NA.25
Grade:
Molecular Biology
Bp:
210 °C (lit.)
Vapor pressure:
0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)
기술 서비스
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도움 문의

Grade

Molecular Biology

Quality Level

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

제품 라인

BioUltra

분석

≥99.5% (T)

양식

liquid

autoignition temp.

932 °F

기술

FISH: suitable
hybridization: suitable
protein extraction: suitable

불순물

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

무기 잔류물

≤0.1%

refractive index

n20/D 1.447 (lit.)
n20/D 1.447

pH

7-9 (25 °C, 1 M in H2O)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

solubility

H2O: 10 M at 20 °C, clear, colorless

density

1.134 g/mL at 25 °C (lit.)

음이온 미량물

chloride (Cl-): ≤1 mg/kg
sulfate (SO42-): ≤10 mg/kg

양이온 미량물

Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH4+: ≤1000 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg

SMILES string

NC=O

λ

10 M in H2O

UV 흡수

λ: 260 nm Amax: ≤0.4
λ: 280 nm Amax: ≤0.1

응용 분야

genomic analysis

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.

애플리케이션

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Formamide has been used:
  • as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
  • in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
  • in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
  • to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
  • as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent

생화학적/생리학적 작용

Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.

특징 및 장점

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

기타 정보

For additional information on our range of Biochemicals, please complete this form.
For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

표적 기관

Blood

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point (°F)

305.6 °F

Flash Point (°C)

152 °C

개인 보호 장비

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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