Name: Guanidine hydrochloride solution
Brand: SIGMA

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About This Item

실험식(Hill 표기법):

CH₅N₃ · HCl

CAS Number:

50-01-1

Molecular Weight:

95.53

Beilstein:

3591990

MDL number:

MFCD00013026

UNSPSC 코드:

12352107

PubChem Substance ID:

57651286

NACRES:

NA.25

기술 서비스

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제품 라인

BioUltra

Quality Level

100

양식

liquid

농도

~8 M in H₂O

불순물

insoluble matter, passes filter test

refractive index

n20/D 1.465

pH

4.5-7.5 (25 °C)

음이온 미량물

sulfate (SO₄^2-): ≤50 mg/kg

양이온 미량물

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤10 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤5 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤150 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV 흡수

λ: 260 nm A_max: 0.040
λ: 280 nm A_max: 0.025

SMILES string

Cl.NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

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애플리케이션

Guanidine hydrochloride solution has been used as a fixation buffer in genomic DNA extraction for genomic analysis.

생화학적/생리학적 작용

Guanidine‐HCl (Gnd-HCl), an efficient chaotropic agent, is broadly used in RNA isolation, globular protein denaturation, and protein refolding studies as a denaturant. It is an appropriate sample buffer for the quick and effective elution of affinity-purified protein complexes and the subsequent protein digestion step. Gnd-HCl is not preferred in proteomics experiments due to its hindrance with trypsin activity at low molar concentrations and tendency to precipitate when combined with sodium dodecyl sulfate (SDS). GnHCl is commonly utilized to extract proteins in extracellular matrix (ECM)‐rich tissues including bone, cartilage, ligament, and tendon.