646563

DL-Dithiothreitol solution

1 M in H₂O

• 동의어(들): (±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
• 실험식(Hill 표기법): C₄H₁₀O₂S₂
• CAS Number: 3483-12-3
• Molecular Weight: 154.25
• Beilstein: 1719757
• MDL number: MFCD00004877
• UNSPSC 코드: 12161504
• PubChem Substance ID: 24883472
• NACRES: NA.25

속성

• 양식: liquid
• Quality Level: 100
• 반응 적합성: reagent type: reductant
• 농도: 1 M in H₂O
• density: 1.04 g/mL at 20 °C; 1.035 g/mL at 25 °C
• SMILES string: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• InChI key: VHJLVAABSRFDPM-QWWZWVQMSA-N

설명

일반 설명

Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.

Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.

애플리케이션

DL-Dithiothreitol solution has been used:

• in western blot analysis
• as a supplement in lysis buffer to study its effects on sperm lysis
• as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
• in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds

특징 및 장점

Low toxicity and reduced odor compared to other thiol compounds.

안전성 정보

• 픽토그램: GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H315,H318
• 예방조치 성명서: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364
• Hazard Classifications: Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter