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일반 설명
TRI reagent® also known as TRIzol, is a monophasic solution of phenol and guanidinium isothiocyanate. It has a potential, to simultaneously solubilize biological material and denatures protein. It is widely used for deproteinizing RNA. This improved version of the single-step total RNA isolation reagent developed by Chomczynski allows a quick, economical, and efficient isolation of total RNA from different biological samples. This product is pure (A260/A280 ~1.8) and can be directly used in Northern blotting. Simultaneous isolation of DNA, RNA and proteins is also possible. 1 mL is sufficient for the extraction of 100 mg tissue or 107 cells.
애플리케이션
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.
TRI Reagent® has been used for isolating RNA from various types of cells or tissues. It may also be used for processing tissues, cells cultured in monolayer, or cell pellets.
특징 및 장점
- Easily scalable RNA isolation
- Works with many sources: human, plant, yeast, bacterial, or viral
- Better yields than traditional guanidine thiocyanate/cesium chloride methods
법적 정보
TRI Reagent is a registered trademark of Molecular Research Center, Inc.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
표적 기관
Nervous system,Kidney,Liver,Skin
보충제 위험성
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 2
Flash Point (°F)
174.2 °F - closed cup
Flash Point (°C)
79 °C - closed cup
개인 보호 장비
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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