A8376

Acylase I from porcine kidney

Grade II, salt-free, lyophilized powder, 300-1,500 units/mg protein

• 동의어(들): Aminoacylase, N-Acylamino acid amidohydrolase
• CAS Number: 9012-37-7
• EC 번호: 3.5.1.14 (BRENDA, IUBMB)
• EC Number: 232-732-3
• MDL number: MFCD00081285
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Grade II
• Quality Level: 200
• 양식: salt-free, lyophilized powder
• 특이 활성도: 300-1,500 units/mg protein
• UniProt 수납 번호: P37111
• 저장 온도: −20°C
• 유전자 정보: pig ... ACY1(396930)

설명

애플리케이션

Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .

생화학적/생리학적 작용

Acylase I is a zinc metalloprotein that catalyzes the kinetic resolution of unnatural and rarely occurring α-amino acids. Its enantioselectivity for the hydrolysis of N-acyl L-α-amino acids is nearly absolute, yet it accepts substrates having a wide range of structure and functionality. Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates.

단위 정의

One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.

분석 메모

Protein determined by biuret.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)