C0371

Anti-Cullin-4A antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-Cul4A
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~89 kDa
• 종 반응성: human
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• 농도: ~1.5 mg/mL
• 기술: indirect immunofluorescence: 10-20 μg/mL using HeLa cells; western blot: 1-2 μg/mL using HEK-293T cells expressing human Cullin-4A
• UniProt 수납 번호: Q13619
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CUL4A(8451)

설명

일반 설명

Cullin-4 (Cul4) proteins are the core components of ubiquitin E3 ligases. Mammalian cells express two distinct but highly homologous cullin-4 proteins, cullin-4A and cullin-4B.

Cullin-4A is a protein encoded by CUL4A gene in humans.

애플리케이션

Anti-Cullin-4A antibody produced in rabbit is used for Immunofluorescence at a concentration of 10-20mg/mL using HeLa cells and is also used for western blotting at a concentration of 1-2μg/mL using HEK-293T cells expressing human Cullin-4A.

생화학적/생리학적 작용

Cullin-4 (Cul4) proteins are components of ubiquitin E3 ligases that regulate cell cycle, genome stability, DNA replication and transcription. CUL4-based E3 ligase plays an important role for the repression of photomorphogenesis in plants (like Arabidopsis thaliana).

Cullin-4A (Cul4A) is involved in identifying specific targets for ubiquitylation. The Cul4A gene is known to be amplified and up-regulated in breast carcinoma and many other tumor types. Cul4A associates with damage-specific DNA binding proteins (DDB1 and DDB2). It functions as substrate recruiting factors for Cul4A-Roc1 ligases. Cul4A is known to ubiquitylate several target proteins including DDB1/DDB2, CDT1 (chromatin licensing and dna replication factor 1), DNA repair protein complementing XP-C cells (XPC), histone H2A, H3 and H4. In response to DNA damage Cul4A complex regulates protein stability of p53 and mouse double minute 2 homolog (MDM2)/ human double minute 2 homolog (HDM2).

물리적 형태

solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)