C0887

Chloroperoxidase from Caldariomyces fumago

buffered aqueous suspension, 1,000-2,000 units/mg protein (E1%/280)

• 동의어(들): Chloride Peroxidase, Chloride:hydrogen-peroxide oxidoreductase
• CAS Number: 9055-20-3
• EC 번호: 1.11.1.10 (BRENDA, IUBMB)
• MDL number: MFCD00130774
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: fungus (Caldariomyces fumago)
• Quality Level: 200
• 양식: buffered aqueous suspension
• 특이 활성도: 1,000-2,000 units/mg protein (E1%/280)
• 분자량: 42 kDa
• 흡수비: RZ ~1.0
• 저장 온도: 2-8°C

설명

애플리케이션

A useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures.

생화학적/생리학적 작용

Chloroperoxidase (CPO) is a 42,000 Da extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group. CPO is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme. It also catalyzes peroxidase-, catalase- and cytochrome P450-type reactions of dehydrogenation, H₂O₂ decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs).

단위 정의

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 °C in the presence of potassium chloride and H₂O₂.

물리적 형태

Purified suspension in 0.1 M sodium phosphate solution, pH approx. 4.5

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)