Product Name
Collagen, Type I solution from rat tail, BioReagent, suitable for cell culture, sterile-filtered
생물학적 소스
rat tail
Quality Level
설명
Approx. 100mg/vial ((35 - 40 ml) )
무균
sterile-filtered
제품 라인
BioReagent
분석
>95% (SDS-PAGE)
양식
liquid
분자량
apparent mol wt 115-130 kDa by SDS-PAGE (doublet)
apparent mol wt 215-235 kDa by SDS-PAGE (doublet)
포장
pkg of 1 vial
농도
>0.5 mg/mL Biuret
기술
cell culture | mammalian: suitable
표면 범위
6‑10 μg/cm2
불순물
Mycoplasma Test by Barile, none detected
solubility
soluble, clear to hazy, colorless (solubilized in 0.02M acetic acid)
NCBI 수납 번호
UniProt 수납 번호
결합 특이성
Peptide Source: Collagen
Peptide Source: Fibronectin
배송 상태
wet ice
저장 온도
2-8°C
유전자 정보
rat ... Col1a1(29393)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Collagen is an extracellular matrix protein comprising triple helical structure. The structure of collagen type I, in particular, is characterized by the presence of three identical α-chains in its homotrimetric form. Among these three α-chains, one exhibits slight sequence variation. Type I collagen was first isolated from rat-tail tendon, has two main α chains (α and α ) and one β chain.
애플리케이션
Collagen, Type I solution from rat tail has been used for coating culture plates and dishes for human hepatocyte HepG2 cell line and podocytes. It has also been used as a standard for total collagen content calibration curve generation for the quantification of liver extracellular matrix pre-gel.
Used as a coating material to support adherent cells growth and differentiation.
생화학적/생리학적 작용
Collagen protein is essential for the mechanical integrity of tendons and bone. Rat tail tendon collagen is used in tissue engineering especially in the generation of 3-D scaffolds based gels. It has low antigenicity and is compatible with human gingival fibroblasts and human oral keratinocytes.
특징 및 장점
- Sourced from rat tail.
- Sterile-filtered to minimize contamination risks.
- Suitable for a wide range of cell culture applications.
- Provides optimal surface coverage (6-10 μg/cm2) for promoting cell adhesion and proliferation.
제조 메모
Recommended for use as a cell culture substratum. May not be suitable for 3-D gel formation.
Sterile solution prepared from rat tail tendons by a modification of the published extraction method 1. It is supplied as an aqueous solution in 20mM acetic acid with a protein concentration as stated on the bottle. (approx.100mg protein per vial). Protein concentration was estimated by the Biuret method 2.
SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample is >95%.
SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample is >95%.
기타 정보
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
When C3867 is first removed from the cooler it will appear gelled. When it is allowed to come fully to 37°C it will appear as a liquid.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.