D5758

Diethyl pyrocarbonate

96% (NT)

• 동의어(들): DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride
• Linear Formula: O(COOC₂H₅)₂
• CAS Number: 1609-47-8
• Molecular Weight: 162.14
• Beilstein: 637031
• EC Number: 216-542-8
• MDL number: MFCD00009106
• UNSPSC 코드: 12352202
• PubChem Substance ID: 24893964
• NACRES: NA.77

속성

• 생물학적 소스: synthetic (microbial fermentation/ cell culture)
• Quality Level: 200
• 분석: 96% (NT)
• 양식: liquid
• refractive index: n20/D 1.398 (lit.)
• bp: 93-94 °C/18 mmHg (lit.)
• density: 1.101 g/mL at 25 °C (lit.)
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• SMILES string: CCOC(=O)OC(=O)OCC
• InChI: 1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
• InChI key: FFYPMLJYZAEMQB-UHFFFAOYSA-N

설명

일반 설명

Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.

애플리케이션

Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

생화학적/생리학적 작용

DEPC is a potent nuclease inhibitor.0.1% solution of DEPC is used to inactive RNase.
It inhibits the ryanodine binding to ryanodine/Ca²⁺ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca²⁺ permeability of SR vesicles.

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302
• 예방조치 성명서: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): 156.2 °F - closed cup
• Flash Point (°C): 69 °C - closed cup
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter