Name: 2′,7′-Dichlorodihydrofluorescein diacetate
Brand: SIGMA

로그인조직 및 계약 가격 보기

About This Item

실험식(Hill 표기법):

C₂₄H₁₆Cl₂O₇

CAS Number:

4091-99-0

Molecular Weight:

487.29

Beilstein:

4610273

MDL number:

MFCD00128955

UNSPSC 코드:

12352200

PubChem Substance ID:

24894058

NACRES:

NA.77

기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

Product Name

2′,7′-Dichlorodihydrofluorescein diacetate, ≥97%

Quality Level

200

분석

≥97%

양식

powder

저장 온도

−20°C

SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O

InChI

1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChI key

PXEZTIWVRVSYOK-UHFFFAOYSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is an oxidation-sensitive fluorescent dye used to detect the intracellular reactive oxygen species (ROS) level. DCFH-DA is a cell-permeable non-fluorescent probe, that can be hydrolyzed by intracellular esterase to DCFH, which forms fluorescent DCF when oxidized by the intracellular ROS.

The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.

애플리케이션

2′,7′-Dichlorofluorescin diacetate is suitable for use in:

sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism
microplate assay for detecting oxidative products in phagocytic cells
quantitative multiwell myeloid differentiation assay

Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.