Each mL of Complete Freund′s adjuvant contains 1 mg of Mycobacterium tuberculosis (H37Ra, ATCC 25177), heat killed and dried, 0.85 mL paraffin oil and 0.15 mL mannide monooleate.

Antigens (preferably in saline) are typically mixed with an equal volume of the adjuvant to form an emulsion.

Freund′s Adjuvants may be used to produce water-in-oil emulsions of immunogens. Antigens in water-in-oil emulsions stimulate high and long-lasting antibody responses which can be attributed to the slow release of antigen.

The mycobacteria in Complete Freund′s adjuvant attracts macrophages and other cells to the injection site which enhances the immune response. For this reason, Complete Freund′s Adjuvant is used for initial injections and Incomplete Freund′s Adjuvant for subsequent boosts.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.