H3506

Hyaluronidase from bovine testes

Type I-S, lyophilized powder, 400-1000 units/mg solid

• 동의어(들): Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• CAS Number: 37326-33-3
• EC 번호: 3.2.1.35 (BRENDA, IUBMB)
• EC Number: 253-464-3
• MDL number: MFCD00131351
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Type I-S
• 양식: lyophilized powder
• 특이 활성도: 400-1000 units/mg solid
• 분자량: ~55 kDa (four subunits of 14 kDa each)
• 기술: cell culture | mammalian: suitable; single cell analysis: suitable
• UniProt 수납 번호: Q5E985; Q8SQG8
• 응용 분야: diagnostic assay manufacturing
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

애플리케이션

Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.

Suitable for use in preparation of single cell suspension for sequencing.

생화학적/생리학적 작용

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

단위 정의

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

기타 정보

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)