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H8163

Sigma-Aldrich

Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-HDAC5

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.41
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기술 서비스
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도움 문의

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

양식

buffered aqueous solution

분자량

antigen ~124 kDa

종 반응성

mouse, human, rat

기술

immunocytochemistry: 1-2 μg/mL using cultured NIH-3T3 or HeLa cells
immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin-embedded human breast carcinoma section
immunoprecipitation (IP): 1-2 μg using extract of 293T cells expressing recombinant mouse HDAC5
microarray: suitable
western blot (chemiluminescent): 2-4 μg/mL using whole extract of human embryonal kidney 293T cells and whole extract of rat pheochromocytoma PC-12 cells

유사한 품목 비교

전체 비교 보기

차이점 표시

1 of 4

이 품목
190047SML1255M1514
assay

99%

assay

99%

assay

≥98% (HPLC)

assay

≥90% (HPLC)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200

mp

161-162 °C (dec.) (lit.)

mp

192 °C (dec.) (lit.)

mp

-

mp

-

일반 설명

Histone Deacetylase 5 (HDAC5) belongs to the class IIa HDAC family. The localization of HDAC5 is both nuclear and cytoplasmic. HDAC5 gene is mapped to human chromosome at 17q21.31.

특이성

Anti-Histone Deacetylase 5 (HDAC5)(NA-16) recognizes human, rat, and mouse HDAC5.

면역원

synthetic peptide corresponding to amino acid residues 384-399 of human HDAC5 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

애플리케이션

Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit has been used in:
  • chromatin immunoprecipitation
  • immunostaining
  • immunocytochemistry
  • immunoprecipitation

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

생화학적/생리학적 작용

Histone Deacetylase 5 (HDAC5) shuttling to the cytoplasm occurs during myogenic differentiation, the nuclear export being stimulated by calcium/ calmodulin-dependent protein kinase (CaMK) phosphorylation at Ser259 and Ser498. HDAC5 activity is important for the differentiation of muscle cells by binding, through its N-terminal domain, to the myocyte enhancer factor-2 (MEF2) protein, thus repressing expression of MEF2 downstream genes.Overexpression of HDAC5 in various cancer cells suppresses their growth by induction of apoptosis in a p53-independent manner. It also plays a key role in many biological processes including the synoviocyte activation, neural regeneration and repair. HDAC5 is highly expressed in breast cancer and contributes to tumor progression.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

C R Ashby et al.
Synapse (New York, N.Y.), 17(3), 173-181 (1994-07-01)
In this study, we examined the interaction of 5-HT1A and 5-HT2A receptors in the rat medial prefrontal cortex (mPFc) using the techniques of extracellular single unit recording and microiontophoresis. The iontophoresis of the selective 5-HT1A receptor agonist (+-)-8-hydroxy-2-(di-n-propylamino) tetralin (8-OHDPAT)
Albrecht Pfäfflin et al.
Annals of clinical biochemistry, 46(Pt 2), 146-148 (2009-01-20)
Serotonin is frequently analysed by high-performance liquid chromatography (HPLC) with electrochemical detection. However, the accuracy of these methods may be affected by the presence of certain drugs. We describe for the first time the interference of paracetamol in therapeutic dosages
Simultaneous micro-determination of serotonin and 5-hydroxyindole-3-acetic acid with 5-hydroxy-N omega-methyltryptamine, as an internal standard, in biological materials by high-performance liquid chromatography with electrochemical detection.
K Shibata et al.
Journal of chromatography, 430(2), 381-387 (1988-09-09)
P M Lalley et al.
The Journal of physiology, 487 ( Pt 3), 653-661 (1995-09-15)
1. The effects of the 5-HT2 receptor agonist alpha-methyl-5-HT were studied on the membrane of expiratory (E2) and post-inspiratory (PI) neurones, by intracellular recordings in the caudal medulla of anaesthetized cats. 2. Ionophoresis of alpha-Me-5-HT depolarized membrane potential and increased
A R Johnston et al.
Experimental brain research, 93(2), 293-298 (1993-01-01)
The effects of 5-hydroxytryptamine (5-HT) and related compounds on the discharge rate of tonically active medial vestibular nucleus (MVN) neurones were studied in an in vitro slice preparation of the dorsal brainstem of the rat. The majority (87 of 107

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