M8787

Magnesium chloride solution

PCR Reagent, 25 mM MgCI₂ solution for PCR

• 동의어(들): PCR optimization
• Linear Formula: MgCl₂
• CAS Number: 7786-30-3
• Molecular Weight: 95.21
• MDL number: MFCD00011106
• UNSPSC 코드: 12352302
• PubChem Substance ID: 57654422
• NACRES: NA.52

속성

• Quality Level: 200; 300
• 양식: liquid
• 포장: vial of 1.5 mL
• 농도: 25 mM±1 mM
• 기술: PCR: suitable
• 색상: colorless
• 응용 분야: agriculture; diagnostic assay manufacturing
• 외래 활성: DNase, RNase, none detected
• 저장 온도: 2-8°C
• SMILES string: Cl[Mg]Cl
• InChI: 1S/2ClH.Mg/h2*1H;/q;;+2/p-2
• InChI key: TWRXJAOTZQYOKJ-UHFFFAOYSA-L

설명

일반 설명

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

애플리케이션

Magnesium chloride solution has been used as a component of the:

• amplification mixture for polymerase chain reaction (PCR)
• amplification mixture in an inter simple sequence repeats (ISSR)-PCR
• reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
• PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
• reaction mix for RT-PCR to assess the gene expression

적합성

Suitable for optimization of polymerase chain reactions.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)