N3129

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

• 동의어(들): p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP
• Linear Formula: O₂NC₆H₄OP(O)(O^-)₂ · 2C₆H₁₁NH₃⁺
• CAS Number: 52483-84-8
• Molecular Weight: 417.44
• Beilstein: 6553276
• EC Number: 257-949-0
• MDL number: MFCD00013173
• UNSPSC 코드: 12352204
• PubChem Substance ID: 24897623
• NACRES: NA.83

속성

• Product Name: 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate
• Quality Level: 300
• 분석: ≥97% anhydrous basis
• 양식: powder
• solubility: water: 10 mg/mL, clear, colorless to faintly yellow
• 저장 온도: −20°C
• SMILES string: NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2
• InChI key: KXNNUYCXODNFBD-UHFFFAOYSA-N

설명

애플리케이션

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

생화학적/생리학적 작용

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)