Product Name
4-Nitrophenyl N-acetyl-β-D-glucosaminide, ≥99% (TLC)
Quality Level
분석
≥99% (TLC)
양식
powder
불순물
≤0.05% Free p-nitrophenol
solubility
water: 5 mg/mL, clear, colorless to very faintly yellow
저장 온도
−20°C
SMILES string
CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc(cc2)[N+]([O-])=O
InChI
1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1
InChI key
OMRLTNCLYHKQCK-DHGKCCLASA-N
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애플리케이션
N-acetyl-β-D-glucosaminidase (NAG) has been used:
- as a substrate to assay N-acetyl-β-D-glucosaminidase (NAG)
- as a substrate to study chitinase ChiB1 activity
- to assess degranulation using rat basophilic leukemia (RBL)-2H3 cells
생화학적/생리학적 작용
4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.
기질
Chromogenic substrate for N-acetyl-glucosaminidase.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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