P3910

Protease from Bacillus licheniformis

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

• 동의어(들): Proteinase from Bacillus licheniformis, Subtilisin A
• CAS Number: 9014-01-1
• EC 번호: 3.4.21.62 (BRENDA, IUBMB)
• EC Number: 232-752-2
• MDL number: MFCD00132092
• UNSPSC 코드: 12352204
• eCl@ss: 32160410
• NACRES: NA.84

속성

• 양식: lyophilized powder
• Quality Level: 100
• 품질: for use in Total Dietary Fiber Assay, TDF-100A
• 분자량: 27 kDa
• 응용 분야: food and beverages
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

특이성

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

애플리케이션

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

생화학적/생리학적 작용

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

물리적 특성

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

단위 정의

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C.

안전성 정보

• 픽토그램: GHS08,GHS05,GHS07,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H318,H334,H335,H410
• 예방조치 성명서: P261 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves