P7173

Pyruvate Carboxylase from bovine liver

buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)

• 동의어(들): Pyruvate:CO₂ ligase (ADP-forming)
• CAS Number: 9014-19-1
• EC 번호: 6.4.1.1 (BRENDA, IUBMB)
• MDL number: MFCD00132143
• UNSPSC 코드: 12352204
• NACRES: NA.26

속성

• 양식: buffered aqueous glycerol solution
• Quality Level: 200
• 특이 활성도: 5-25 units/mg protein (BCA)
• 농도: ≥0.5 mg/mL
• 외래 활성: lactic dehydrogenase ≤0.5%
• 저장 온도: −20°C

설명

애플리케이션

Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.

The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.

생화학적/생리학적 작용

Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.

단위 정의

One unit will convert 1.0 μmole of pyruvate and CO₂ to oxalacetate per min at pH 7.8 at 30 °C.

물리적 형태

Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.

제조 메모

Affinity purified

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable