Name: Puromycin dihydrochloride from <I>Streptomyces alboniger</I>
Brand: SIGMA

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About This Item

실험식(Hill 표기법):

C₂₂H₂₉N₇O₅ · 2HCl

CAS Number:

58-58-2

Molecular Weight:

544.43

Beilstein:

3853613

EC Number:

200-387-8

MDL number:

MFCD00012691

UNSPSC 코드:

12352207

PubChem Substance ID:

24898984

NACRES:

NA.76

기술 서비스

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도움 문의

Product Name

Puromycin dihydrochloride from Streptomyces alboniger, powder, BioReagent, suitable for cell culture

생물학적 소스

Streptomyces alboniger

Quality Level

300

제품 라인

BioReagent

분석

≥98% (HPLC)

양식

powder

분자량

544.43 g/mol

포장

pkg of 10 mg
pkg of 100 mg
pkg of 25 mg

환경친화적 대안 제품 점수

old score: 88
new score: 79
Find out more about DOZN™ Scoring

환경친화적 대안 제품 특성

Atom Economy
Design for Energy Efficiency
Use of Renewable Feedstocks
Design for Degradation
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

기술

cell culture | mammalian: suitable

색상

yellow-white

UniProt 수납 번호

P13249

항생제 활성 스펙트럼

Gram-positive bacteria
neoplastics
parasites

응용 분야

agriculture

환경친화적 대안 카테고리

Re-engineered,

동작 모드

protein synthesis | interferes

저장 온도

−20°C

SMILES string

Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1

InChI

1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1

InChI key

MKSVFGKWZLUTTO-FZFAUISWSA-N

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일반 설명

Chemical structure: peptidyl nucleoside

Puromycin is a broad spectrum antibiotic produced by Streptomyces alboniger. It is an analog of the 3′ end of aminoacyl-tRNA. It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes.

We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Atom Economy”, “Design for Energy Efficiency”, “Use of Renewable Feedstock” and “Design for Degradation”. Click here to view its DOZN scorecard.

애플리케이션

Puromycin dihydrochloride from Streptomyces alboniger has been used:

in the preparation of puromycin stock solution for puromycin sensitivity assay.
to select lentivirus-infected cells containing puromycin resistant selection marker.

Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.

Used as a selective agent for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). Recommended for use at a range of 1-10 μg/ml. Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.

생화학적/생리학적 작용

Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA.

Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.

Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.

Puromycin associates in a non-specific manner with growing polypeptide chains, and thus, leads to premature termination of translation. It inhibits protein synthesis in two ways. It associates with the donor substrate, peptidyl-tRNA, in the P site and thus, functions as an acceptor substrate. Second, it competes with aminoacyl tRNA to bind with the A′ site within the peptidyl transferase center.

주의사항

As supplied, this product remains active for four years when stored at -20°C.

제조 메모

This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.

기타 정보

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place

픽토그램

GHS07

신호어

Warning

유해 및 위험 성명서

H302

예방조치 성명서

P301 + P312 + P330

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves

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