R4526

Reference Dye for Quantitative PCR

100 ×, solution

• 동의어(들): Reference dye for qPCR, ROX
• UNSPSC 코드: 41106300
• NACRES: NA.55

속성

• 양식: solution
• Quality Level: 300
• 사용: sufficient for ≥600 reactions
• 농도: 100 ×
• 기술: PCR: suitable
• 색상: red
• solubility: water: soluble
• 저장 온도: 2-8°C

설명

일반 설명

Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

애플리케이션

Reference Dye for Quantitative PCR has been used:

• in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
• as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
• for analyzing the degree of cellular DNA contamination by qPCR

기타 정보

Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.

안전성 정보

• 픽토그램: GHS07,GHS09
• 신호어: Warning
• 유해 및 위험 성명서: H315,H317,H319,H410
• 예방조치 성명서: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves