SRP0417

BirA

recombinant, expressed in E. coli, ≥65% (SDS-PAGE)

• 동의어(들): BirA Biotin Ligase
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• 분석: ≥65% (SDS-PAGE)
• 양식: aqueous solution
• 분자량: 37 kDa
• 포장: pkg of 100 μg
• NCBI 수납 번호: AP012306
• 배송 상태: dry ice
• 저장 온도: −70°C

설명

일반 설명

Biotin ligase BirA (GenBank Accession No. AP012306 (3602298-3603260)) amino acids 2 – 321 (end) with His-FLAG-tag, MW = 37.1 kDa, expressed in an Escherichia coli cell expression system.

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

생화학적/생리학적 작용

Biotin ligase BirA controls biotin synthesis in Escherichia coli. It sends biotin to metabolism. As a homodimer, it negatively controls the biotin synthetic operon. BirA also catalyzes post-translational biotinylation, by associating with biotin acceptor protein of acetyl-coenzyme A carboxylase. It can biotinylate human histones.

물리적 형태

Formulated in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% Glycerol.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H360D
• 예방조치 성명서: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable