Product Name
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L43
제품 라인
Discovery®
특징
endcapped
제조업체/상표
Discovery®
포장
1 ea of
라벨링 범위
12% Carbon loading
파라미터
≤70 °C temp. range
400 bar pressure (5801 psi)
기술
HPLC: suitable
LC/MS: suitable
길이 × I.D.
15 cm × 4.6 mm
표면적
300 m2/g
표면 범위
4 μmol/m2
불순물
<10 ppm metals
Matrix
silica gel, high purity, spherical particle platform
fully porous particle
기질 활성군
PFP (pentafluorophenyl) phase
입자 크기
3 μm
공극 크기
120 Å
작동 pH 범위
2-8
응용 분야
food and beverages
분리 기술
reversed phase
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
애플리케이션
- Nature of the main contaminant in the drug primaquine diphosphate: SFC and SFC-MS methods of analysis.: This study uses the Discovery® HS F5 HPLC Column to identify and quantify the main contaminant in primaquine diphosphate, crucial for ensuring the quality and safety of this antimalarial drug (Brondz et al., 2007).
- HPLC assisted chemobiological standardization of alpha-glucosidase-I enzyme inhibitory constituents from Piper longum Linn-An Indian medicinal plant.: Demonstrates the application of the Discovery® HS F5 HPLC Column in the biologically relevant chemical analysis of Indian medicinal plants, contributing to the standardization of herbal medicine (Pullela et al., 2006).
- Disposition study of a new potential antineoplastic agent dimefluron in rats using high-performance liquid chromatography with ultraviolet and mass spectrometric detection.: Utilizes the Discovery® HS F5 HPLC Column to study the pharmacokinetics and metabolism of a novel anticancer agent, aiding in the development of effective cancer therapies (Císar et al., 2005).
- A simplified analytical method for a phenotyping cocktail of major CYP450 biotransformation routes.: This research employs the Discovery® HS F5 HPLC Column to simplify the analysis of a phenotyping cocktail, enhancing the assessment of drug metabolism and facilitating personalized medicine approaches (Jerdi et al., 2004).
특징 및 장점
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
법적 정보
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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