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Merck

The Expression of Transcription Factors in Fetal Lamb Kidney.

Journal of developmental biology (2021-07-03)
Yuri Nishiya, Kohei Kawaguchi, Kosuke Kudo, Takuya Kawaguchi, Juma Obayashi, Kunihide Tanaka, Kei Ohyama, Hideki Nagae, Shigeyuki Furuta, Yasuji Seki, Junki Koike, Kevin C Pringle, Hiroaki Kitagawa
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(1) Background: Renal development involves frequent expression and loss of transcription factors, resulting in the activation of genes. Wilms' tumor 1 (WT1), hepatocyte nuclear factor-1-beta (HNF1ฮฒ), and paired box genes 2 and 8 (Pax2 and Pax8) play an important role in renal development. With this in vivo study, we examined the period and location of expression of these factors in renal development. (2) Methods: Fetal lamb kidneys (50 days from gestation to term) and adult ewe kidneys were evaluated by hematoxylin and eosin staining. Serial sections were subjected to immunohistochemistry for WT1, HNF1ฮฒ, Pax2, and Pax8. (3) Results: Pax2, Pax8, and HNF1ฮฒ expression was observed in the ureteric bud and collecting duct epithelial cells. We observed expression of WT1 alone in metanephric mesenchymal cells, glomerular epithelial cells, and interstitial cells in the medullary rays and Pax8 and HNF1ฮฒ expression in tubular epithelial cells. WT1 was highly expressed in cells more proximal to the medulla in renal vesicles and in C- and S-shaped bodies. Pax2 was expressed in the middle and peripheral regions, and HNF1ฮฒ in cells in the region in the middle of these. (4) Conclusions: WT1 is involved in nephron development. Pax2, Pax8, and HNF1ฮฒ are involved in nephron maturation and the formation of peripheral collecting ducts from the Wolffian duct.

MATERIALS
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Sigma-Aldrich
Anti-HNF1B antibody produced in rabbit, Prestige Antibodiesยฎ Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution