์ฝ˜ํ…์ธ ๋กœ ๊ฑด๋„ˆ๋›ฐ๊ธฐ
Merck

A BEN-domain-containing protein associates with heterochromatin and represses transcription.

Journal of cell science (2011-09-15)
Kizhakke M Sathyan, Zhen Shen, Vidisha Tripathi, Kannanganattu V Prasanth, Supriya G Prasanth
์ดˆ๋ก

In eukaryotes, higher order chromatin structure governs crucial cellular processes including DNA replication, transcription and post-transcriptional gene regulation. Specific chromatin-interacting proteins play vital roles in the maintenance of chromatin structure. We have identified BEND3, a quadruple BEN domain-containing protein that is highly conserved amongst vertebrates. BEND3 colocalizes with HP1 and H3 trimethylated at K9 at heterochromatic regions in mammalian cells. Using an in vivo gene locus, we have been able to demonstrate that BEND3 associates with the locus only when it is heterochromatic and dissociates upon activation of transcription. Furthermore, tethering BEND3 inhibits transcription from the locus, indicating that BEND3 is involved in transcriptional repression through its interaction with histone deacetylases and Sall4, a transcription repressor. We further demonstrate that BEND3 is SUMOylated and that such modifications are essential for its role in transcriptional repression. Finally, overexpression of BEND3 causes premature chromatin condensation and extensive heterochromatinization, resulting in cell cycle arrest. Taken together, our data demonstrate the role of a novel heterochromatin-associated protein in transcriptional repression.

MATERIALS
Product Number
๋ธŒ๋žœ๋“œ
์ œํ’ˆ ์„ค๋ช…

Sigma-Aldrich
Anti-HP1ฮฑ Antibody, clone15.19s2, clone 15.19s2, Upstateยฎ, from mouse
SKU
ํŒฉ ํฌ๊ธฐ
์žฌ๊ณ  ์ •๋ณด
๊ฐ€๊ฒฉ
์ˆ˜๋Ÿ‰
Millipore
Human C-Peptide ELISA, This Human C-Peptide ELISA is used to measure & quantify C-Peptide levels in Metabolism & Endocrine research.
SKU
ํŒฉ ํฌ๊ธฐ
์žฌ๊ณ  ์ •๋ณด
๊ฐ€๊ฒฉ
์ˆ˜๋Ÿ‰
Sigma-Aldrich
Benzonaseยฎ Nuclease, ultrapure, โ‰ฅ250 units/ฮผL, โ‰ฅ99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
SKU
ํŒฉ ํฌ๊ธฐ
์žฌ๊ณ  ์ •๋ณด
๊ฐ€๊ฒฉ
์ˆ˜๋Ÿ‰
Sigma-Aldrich
Anti-PCNA Antibody, clone PC10, clone PC10, Chemiconยฎ, from mouse
SKU
ํŒฉ ํฌ๊ธฐ
์žฌ๊ณ  ์ •๋ณด
๊ฐ€๊ฒฉ
์ˆ˜๋Ÿ‰
Millipore
Benzonaseยฎ Nuclease, โ‰ฅ250 units/ฮผL, โ‰ฅ90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
SKU
ํŒฉ ํฌ๊ธฐ
์žฌ๊ณ  ์ •๋ณด
๊ฐ€๊ฒฉ
์ˆ˜๋Ÿ‰