HPLC Analysis of Bupivacaine Enantiomers on Astecยฎ CHIROBIOTICยฎ V2, Formate Mobile Phase

Materials
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CONDITIONS
column
CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)
mobile phase
[A] 20 mM ammonium formate, pH 4.1; [B] methanol; (5:95, A:B)
flow rate
1 mL/min
column temp.
20 ยฐC
detector
UV, 220 nm
injection
5 μL
sample
1 mg/mL in methanol
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Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC