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Merck

HPLC Analysis of Phenolics from Rosemary on Ascentisยฎ Express C8

HPLC Analysis of Phenolics from Rosemary on Ascentis® Express C8 application for HPLC

Materials

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Ascentisยฎ Express C8, 2.7 ฮผm HPLC Column

2.7 μm particle size, L ร— I.D. 5 cm ร— 3 mm

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Carnosic acid

analytical standard

Carnosol

analytical standard

Rosmarinic acid

โ‰ฅ98% (HPLC), from Rosemarinus officinalis L.

CONDITIONS

column

Ascentis Express C8, 5 cm x 3 mm I.D., 2.7 μm particles (53848-U)

mobile phase

[A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile

gradient

15 to 100% B in 8.5 min

flow rate

0.6 mL/min

column temp.

35 ยฐC

detector

UV, 214 nm

injection

2 μL

sample

100 mg/L in 75 μM EDTA, 1.5 mM ascorbate, 15% acetonitrile

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This application demonstrates the suitability of Ascentis Express C8 for the efficient separation of phenolics from rosemary.

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Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany