HPLC Analysis of Phenolics from Rosemary on Ascentisยฎ Express C8

Materials
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CONDITIONS
column
Ascentis Express C8, 5 cm x 3 mm I.D., 2.7 μm particles (53848-U)
mobile phase
[A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient
15 to 100% B in 8.5 min
flow rate
0.6 mL/min
column temp.
35 ยฐC
detector
UV, 214 nm
injection
2 μL
sample
100 mg/L in 75 μM EDTA, 1.5 mM ascorbate, 15% acetonitrile
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This application demonstrates the suitability of Ascentis Express C8 for the efficient separation of phenolics from rosemary.
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Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany