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Merck

LC/MS Analysis of Cyanuric Acid on Ascentisยฎ Express HILIC

LC/MS Analysis of Cyanuric Acid on Ascentis® Express HILIC application for HPLC

Materials

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53934-U

Ascentisยฎ Express HILIC, 2.7 ฮผm HPLC Column

2.7 μm particle size, L ร— I.D. 5 cm ร— 2.1 mm

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70221

Ammonium formate

eluent additive for LC-MS, LiChropurโ„ข, โ‰ฅ99.0%

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16614

Cyanuric acid

analytical standard

49866

Ammelide

analytical standard

CONDITIONS

column

Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.7 μm particles (53934-U)

mobile phase

5 mM ammonium formate in 95:5 (v/v) acetonitrile:water

flow rate

0.2 mL/min

pressure

328 psi (22.6 bar)

column temp.

35 ยฐC

detector

MS, ESI(-), SIR mode

injection

2 μL

sample

1 μg/mL in mobile phase

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Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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