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About This Item

Empirical Formula (Hill Notation):
C20H21N3O5S
CAS Number:
16650-83-2
Molecular Weight:
415.46
UNSPSC Code:
41116130
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Quality Level

400

form

solid

composition

Dye, ≥90% spectrophotometric assay

pH

2.2 (20 °C, 10 g/L in H2O)

mp

>300 °C

solubility

50 g/L

bulk density

300 kg/m3

εmax

1630-1820 at 635-639 nm in 50% ethanol at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C20H20N3O.H2O4S/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h5-12H,3-4,21H2,1-2H3;(H2,1,2,3,4)/q+1;/p-1

InChI key

MMYKGGIXVBGGSZ-UHFFFAOYSA-M

General description

Nile blue (hydrogen sulfate) (C.I. 51180) is a CERTISTAIN dry dye that is used for the preparation of a staining solution e.g. staining melanins and lipofuscins in histological sections of human origin.
As CERTISTAIN dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity
- Hydrogen sulfate (as sulfur):
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 635 - 639 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 1630 - 1820
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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