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Btri-C3-PAA-biot

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UNSPSC Code:
12352201
NACRES:
NA.25
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carrier

PAA carrier

conjugate

biotin conjugate

product line

BP-Series

Assay

95% (H-NMR)

form

lyophilized powder

mol wt

~20,000 Da

manufacturer/tradename

GlycoNZ

extent of labeling

20 mol% Biotin ligand density

technique(s)

flow cytometry: suitable
glycol-bead construction: suitable
glycol-surface construction: suitable

probe type

trisaccharide

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This Item
0007-BP0085-BP0048-BP
conjugate

biotin conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

probe presentation

polymeric

probe presentation

polymeric

probe presentation

polymeric

probe presentation

polymeric

probe type

trisaccharide

probe type

tetrasaccharide

probe type

trisaccharide

probe type

disaccharide

carrier

PAA carrier

carrier

PAA carrier

carrier

PAA carrier

carrier

PAA carrier

technique(s)

flow cytometry: suitable, glycol-surface construction: suitable, glycol-bead construction: suitable

technique(s)

flow cytometry: suitable, glycol-bead construction: suitable, glycol-surface construction: suitable

technique(s)

flow cytometry: suitable, glycol-bead construction: suitable, glycol-surface construction: suitable

technique(s)

flow cytometry: suitable, glycol-bead construction: suitable, glycol-surface construction: suitable

product line

BP-Series

product line

BP-Series

product line

BP-Series

product line

BP-Series

General description

BP-Series: Biotinylated Polymeric Probes
This series of glycoconjugate probes includes HOCH2CH2NH3+-salts for acidic sugars. The affinity of these probes is 102 to 105 times higher than that of the corresponding free sugars. The spacer-arm is normally –(CH2)3–, while the spacer arm for biotin is –(CH2)6–. The flexible polymer chain behaves as an additional spacer.

Used as a high molecular weight carrier, poly[N-(2-hydroxyethyl)acrylamide], abbreviated as PAA, has
low non-specific sorption, and is stable to chemical and proteolytic action.

Application

Glycoconjugates are used to study the functions of carbohydrates, carbohydrate-binding proteins in biological processes, and on the discovery of new carbohydrate-mediated interactions.

Preparation Note

Purity: synthetic ω-aminoalkyl glycosides used for coupling with the polymer have a purity of >95% (HPLC and 1H-NMR)

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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The biotin-(strept)avidin system: principles and applications in biotechnology.
Diamandis EP and Christopoulos TK
Clinical Chemistry, 37, 625-625 (1991)
Isolation and culture of rat and mouse oligodendrocyte precursor cells.
Chen Y et al.
Nature Protocols, 2, 1044-1044 (2007)
Lone Frydelund-Larsen et al.
Experimental physiology, 92(1), 233-240 (2006-10-13)
Exercise induces a marked increase in interleukin-8 (IL-8) mRNA and protein expression within skeletal muscle fibres. Interleukin-8 belongs to a subfamily of CXC chemokines containing a Glu-Leu-Arg (ELR) motif. CXC chemokines with ELR motifs are potent angiogenic factors in vivo
Michael T Jacobsen et al.
Cell chemical biology, 24(8), 1040-1047 (2017-08-02)
Chemical modification of proteins provides great opportunities to control and visualize living systems. The most common way to modify proteins is reaction of their abundant amines with N-hydroxysuccinimide (NHS) esters. Here we explore the impact of amine number and positioning on
Christopher Ritchie et al.
Cancer genomics & proteomics, 14(4), 225-239 (2017-06-26)
Mutations of the human K-Ras 4B (K-Ras) G protein are associated with a significant proportion of all human cancers. Despite this fact, a comprehensive analysis of K-Ras interactions is lacking. Our investigations focus on characterization of the K-Ras interaction network.

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