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B0904

Sigma-Aldrich

Basic Fuchsin

certified by the Biological Stain Commission, powder

Synonym(s):

Basic Red 9, Fuchsin basic, Magenta O, Pararosaniline

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About This Item

UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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Product Name

Basic Fuchsin, special for flagella, certified

Agency

certified by the BSC

Quality Level

type

special for flagella, certified

form

powder

composition

pararosaniline acetate, 3 parts
pararosaniline chloride, 1 part

technique(s)

microbe id | staining: suitable

color

green to very dark green

pH

5.0-6.0 (1 g/L)

mp

250 °C

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General description

Basic fuchsin, a triphenylmethane dye is commonly a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II and new fuchsine (CI 42520). Basic fuchsin is widely used in tissue-specific staining of biological and coloring several materials including orlon, cotton, leather, paper etc. It is also used to stain collagen, muscle and mitochondria.

Application

Basic Fuchsin has been used in several biological tests including:
  • Acid-fast staining with carbol-fuchsine
  • Periodic Acid-Schiff method
  • Feulgen technique for DNA staining
  • ENDO culture medium for distinguishing between the bacteria Escherichia coli and Aerobacter aerogenes
  • Leifson′s method for bacterial flagella staining.

Biochem/physiol Actions

Basic fuchsin possesses anesthetic, bactericidal (gram-positive), and fungicidal properties.

Suitability

Certified for use in Leifson′s flagella stain.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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