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About This Item
Empirical Formula (Hill Notation):
C19H18N4O4
CAS Number:
Molecular Weight:
366.37
MDL number:
UNSPSC Code:
12352108
PubChem Substance ID:
NACRES:
NA.32
Quality Level
Assay
≥98.0% (HPLC)
form
powder
solubility
DMF: soluble
DMSO: soluble
fluorescence
λex 428 nm; λem 453 nm
storage temp.
−20°C
SMILES string
CN(C)c1ccc(cc1)\N=N\c2ccc(cc2)C(=O)ON3C(=O)CCC3=O
CN(C)c1ccc(cc1)\N=N\c2ccc(cc2)C(=O)ON3C(=O)CCC3=O
InChI
1S/C19H18N4O4/c1-22(2)16-9-7-15(8-10-16)21-20-14-5-3-13(4-6-14)19(26)27-23-17(24)11-12-18(23)25/h3-10H,11-12H2,1-2H3/b21-20+
InChI key
IBOVDNBDQHYNJI-QZQOTICOSA-N
Application
A useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups by forming stable peptide bonds. The succinimidyl ester is reactive with terminal amines or lysines of peptides and other nucleophiles for fluorescent studies of proteins. Its fluorescent properties (has a characteristic broad and intense visible absorption but has no fluorescence) make it an ideal long wavelength quencher and it has been utilized as an acceptor chromophore in FRET studies.
Analysis Note
Absorption spectra shows a maximum at 453 nm (in methanol). After reaction with butylamine: max. absorption at 428 nm (Lit.)
Other Notes
N-terminal modification of peptides in automated synthesis
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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