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About This Item
Empirical Formula (Hill Notation):
C6H2ClN3O3
CAS Number:
Molecular Weight:
199.55
Beilstein:
614212
EC Number:
MDL number:
UNSPSC Code:
12352100
PubChem Substance ID:
NACRES:
NA.32
product line
BioReagent
Quality Level
Assay
≥97.0% (HPLC)
mp
97-100 °C
97-99 °C (lit.)
fluorescence
λex 336 nm in methanol
λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)
suitability
suitable for fluorescence
SMILES string
[O-][N+](=O)c1ccc(Cl)c2nonc12
InChI
1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
InChI key
IGHBXJSNZCFXNK-UHFFFAOYSA-N
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Application
4-Chloro-7-nitrobenzofurazan (NBD-chloride) is a fluorescence assay reagent used to label free sulfhydryls and N-terminals within proteins. NBD-chloride is used as an enzyme inhibitor and as a trapping agent for cysteine sulfenic acid.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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