Sign Into View Organizational & Contract Pricing
About This Item
Linear Formula:
C21H17ClN2O3
CAS Number:
Molecular Weight:
380.82
Beilstein:
6030951
EC Number:
MDL number:
UNSPSC Code:
12352116
PubChem Substance ID:
NACRES:
NA.32
product line
BioReagent
Quality Level
Assay
≥85% (HPLC)
form
powder
impurities
≤10% water
solubility
ethanol: 20 mg/mL, clear, red
suitability
in accordance for fluorescence
suitable for fluorescence
SMILES string
Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24
InChI
1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H
InChI key
MYFATKRONKHHQL-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
Rhodamine 123 is a fluorescent dye.
Application
Rhodamine 123 has been used:
- as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
- to label the cells for estimating the P-gp expression by flow cytometry
- in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
- the detection of mitochondrial membrane potential (ΔΨm)
Biochem/physiol Actions
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.
Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.
Other Notes
Supravital mitochondrial-specific dye
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service