grade
Molecular Biology
Quality Level
form
liquid
usage
75 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s DirectLoad™ PCR 100 bp Low Ladder contains 10 dsDNA fragments from 100 − 1,000 bases, evenly spaced in 100 bp repeats. Ladder should be loaded in a single lane on an agarose gel.
Application
Suitable for size determination of PCR generated DNA fragments with either agarose or polyacrylamide gels.
Features and Benefits
- Ready-to-load
- Easy-to-use
- Popular band sizes
Components
Sigma′s DirectLoad™ PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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