Name: Bovine Fibronectin
Brand: SIGMA

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About This Item

EC Number:

289-149-2

MDL number:

MFCD00131062

UNSPSC Code:

12352202

eCl@ss:

42020141

NACRES:

NA.75

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Product Name

Fibronectin bovine plasma, powder, BioReagent, suitable for cell culture

biological source

bovine plasma

Quality Level

200

sterility

sterile

product line

BioReagent

form

powder

mol wt

~450 kDa

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

concentration

≥1 mg protein/mL

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm²

solubility

H₂O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

web metadata keyword.default

attachment factor, mesenchymal cell attachment, neuronal cell attachment, fibroblast attachment, neural crest cell attachment, endothelial cell attachment

NCBI accession no.

NM_001163778.1

UniProt accession no.

P07589

shipped in

dry ice

storage temp.

−20°C

Gene Information

bovine ... FN1(2807940)

General description

Fibronectin is a glycoprotein with multiple domains. It comprises a N-terminal, central binding domain and a heparin-binding domain. Bovine plasma fibronectin has polypeptide chains with disulfide linkage and corresponds to a molecular weight of 220 and 215 kDa.

Fibronectins are adhesive glycoproteins and are found both as cell surface proteins and in plasma. Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.

Application

Fibronectin from bovine plasma is used for the following applications:

Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)
Cell movement assays (culture plates were coated with fibronectin)
Cell spreading and immunofluorescence
Protein specificity
Integration of sensor films into microfluidic chips
Histological and Immunohistochemical (IHC) analyses
Used in primary cell cultures from patients with lung carcinoma

Biochem/physiol Actions

Fibronectin is a regulator of protein scaffolding. It coordinates cellular processes like platelet functions, wound-healing, adhesion, migration and aggregation.Study shows that human dental pulp (HDP) cells rely on fibronectin (FN) for initial attachment and subsequent spreading. FN may exhibit mitogenic activity, promoting cell proliferation, and also play a role in regulating the differentiation process of pulp cells into odontoblasts. The presence of FN appears to influence the fate and development of HDP cells, specifically in their differentiation into odontoblasts, which are responsible for dentin formation in teeth.

Caution

As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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