recombinant
expressed in E. coli
Quality Level
sterility
sterile; autoclaved
product line
BioReagent
form
powder
mol wt
~110 kDa by SDS-PAGE
packaging
pkg of 1 mg
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 1 mg/mL (stable for 12 mo. at RT)
web metadata keyword.default
attachment factor, mesenchymal cell attachment, neuronal cell attachment, fibroblast attachment, neural crest cell attachment, endothelial cell attachment
shipped in
ambient
storage temp.
room temp
Application
Recommended for use as a cell culture substratum
at 2-10 μg/cm2.
at 2-10 μg/cm2.
Other Notes
Positively-charged, protein polymer which incorporates multiple copies of the RGD cell attachment epitope from human fibronectin between repeated structural peptide units.
Linkage
Similar to F5022 but more highly charged polymer which encourages better adhesion in many cell lines.
Reconstitution
Dilutions can be made to a working concentration with balanced salt solution; stable up to 2 days at RT. Coated labware is stable at least 4 months at RT.
Legal Information
ProNectin is a registered trademark of Sanyo Chemical Industries, Ltd
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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