grade
Molecular Biology
Quality Level
form
liquid
usage
80 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
2-8°C
General description
Sigma′s PCR 20 bp Low Ladder contains 50 dsDNA fragments from 50 – 1,000 bases, evenly spaced in 20 bp repeats. Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
Application
PCR 20 bp Low Ladder has been used as a marker for DNA gel electrophoresis.
Components
Sigma′s PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Other Notes
Contains 50 bands, 20–1,000 bp in exact 20 bp repeats.
For optimal resolution, the recommended agarose gel concentration is 2.5%.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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