Quality Level
form
liquid
usage
sufficient for 50 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s PCR 50 - 2,000 bp Marker contains 8 dsDNA fragments from 50 – 2,000 bases, evenly spaced. Five μl of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.
Application
PCR 50 - 2,000 bp Marker has been used as a marker for DNA gel electrophoresis.
Components
Sigma′s PCR 50 - 2,000 bp Marker is provided as two tubes:
- 1 vial (250 μl volume) PCR Marker
- 1 vial 6× loading buffer (250 μl volume) for diluting samples
Other Notes
Contains 8 bands, 50–2,000 bp in predictably spaced (ladder) double-stranded DNA fragments.
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Storage Class Code
10 - Combustible liquids
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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