A1164

Azaserine

Hybri-Max^™, γ-irradiated, 50x, lyophilized powder, BioXtra, suitable for hybridoma

• Synonym(s): O-Diazoacetyl-L-serine
• Empirical Formula (Hill Notation): C₅H₇N₃O₄
• CAS Number: 115-02-6
• Molecular Weight: 173.13
• Beilstein: 1726602
• EC Number: 204-061-6
• MDL number: MFCD00036802
• UNSPSC Code: 12352207
• PubChem Substance ID: 24890511
• NACRES: NA.75

Properties

• grade: Hybri-Max^™
• Quality Level: 200
• sterility: γ-irradiated
• product line: BioXtra
• form: lyophilized powder
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested
• antibiotic activity spectrum: fungi
• Mode of action: enzyme | inhibits
• storage temp.: −20°C

Description

General description

Chemical structure: amino acid derivatives

Application

For use in hybridoma cell culture applications as a myeloma selection agent. Used to study inhibition of formylglycinamide ribonucleotide amidotransferase and PRPP amidotransferase.

Biochem/physiol Actions

Azaserine is an antibiotic, generated by Streptomyces fragilis.[1] It is considered as a potential mutation inducing agent.[2] It is known to promote pancreatic cancer and induces adenoma and carcinoma in vivo and in vitro. Azaserine also stimulates kidney and liver tumors in rats.[1]

Reconstitution

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500 mL medium. Final working concentration: 5.7 μM azaserine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H301,H351
• Precautionary Statements: P201 - P301 + P310 + P330
• Hazard Classifications: Acute Tox. 3 Oral - Carc. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves