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A2230

Sigma-Aldrich

Apyrase from potatoes

High Activity, ATPase ≥600 units/mg protein, lyophilized powder

Synonym(s):

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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1 G
2 053,20 MYR

2 053,20 MYR

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1 G
2 053,20 MYR

About This Item

CAS Number:
9000-95-7
EC Number:
3.6.1.5 (BRENDA, IUBMB)
MDL number:
MFCD00130542
UNSPSC Code:
12352204
NACRES:
NA.54

2 053,20 MYR

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biological source

potato

Quality Level

300

form

lyophilized powder

quality

High Activity

ATPase activity

≥600 units/mg protein

secondary activity

≥50 % of base activity ADPase

composition

protein, ≥30%

foreign activity

Acid Phosphatase ≤2% of base activity

shipped in

wet ice

storage temp.

−20°C

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material

black

material

blue

material

white polystyrene

material

white polystyrene

manufacturer/tradename

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manufacturer/tradename

Bel-Art F13374-0002

manufacturer/tradename

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manufacturer/tradename

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Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation [1]. Product A2230 is a high activity apyrase.
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis [2]. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Packaging

Sold on the basis of ATPase units.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Physical form

Lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Derived from red potato

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCH5984
SLBT6460
SLBS0654V
SLBN5704V
SLBD6482

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