biological source
goat
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
human
technique(s)
direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... IGK@(50802)
General description
Human κ light chains are antibody fragments that regulate immunological responses in cells. Increased expression of κ light chains has been linked to chronic lymphocytic leukemia . The variable fragment of the κ light chain gene has also been associated with vaccine-induced antibody response to the polysaccharide coat in Haemophilus influenzae type b . Anti-Human κ Light Chains (Bound and Free-Alkaline Phosphatase antibody is specific for human κ light chain when tested against human IgA, IgG, IgM, IgGλ, IgAλ, IgMλ, Bence Jones κ and λ myeloma proteins.
Immunogen
Purified Bence Jones κ light chain
Application
Anti-Human κ Light Chains (Bound and Free)-Alkaline Phosphatase antibody is suitable for use in ELISA. The product can also be used for dot blot (1:30,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Mouse-Human chimeric IgG′s specific for Pseudomonas aeruginosa serogroup 06 lipopolysaccharide were identified by ELISA in transfected CHO cell supernatants using Alkaline phosphatase conjugated goat anti-Human Kappa Light chains.
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service