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C3657

Sigma-Aldrich

Human Collagen Type V

from human placenta, powder, suitable for detection assays

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500 ML
₹4,310.00

About This Item

CAS Number:
9007-34-5
EC Number:
232-697-4
MDL number:
MFCD00130825
UNSPSC Code:
12352202
NACRES:
NA.32

₹4,310.00

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Product Name

Collagen from human placenta, Bornstein and Traub Type V (Sigma Type IX), powder

biological source

human placenta

Quality Level

200

form

powder

impurities

HIV, hepatitis B and hepatitis C, none detected

solubility

aqueous acid: soluble

UniProt accession no.

P20908

application(s)

detection

storage temp.

2-8°C

Gene Information

human ... COL5A1(1289)

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.

Caution

This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.

Preparation Note

This product was prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.

Analysis Note

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

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Polycarbonate Membrane Filter, 0.6 μm Pore Size Isopore™, filter diam. 13 mm, hydrophilic

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Polycarbonate Membrane Filter, 0.22 μm Pore Size Isopore™, filter diam. 13 mm, hydrophilic, white

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Polycarbonate Membrane Filter, 0.8 μm Pore Size Isopore™, filter diam. 37 mm, hydrophilic

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Polycarbonate Membrane Filter, 0.8 μm Pore Size

manufacturer/tradename

Isopore™, Millipore

manufacturer/tradename

Isopore™, Millipore

manufacturer/tradename

Isopore™, Millipore

manufacturer/tradename

Isopore™, Millipore

parameter

140 °C max. temp., ~43 mL/min-cm2 water flow rate at 10 psi (typical results), 10 L/min-cm2-psi air flow rate, 62 mL/min-cm2-psi water flow rate (maximum flow rate per 1.0 psi)

parameter

10 L/min-cm2-psi air flow rate, 140 °C max. temp., 62 mL/min-cm2-psi water flow rate (maximum flow rate per 1.0 psi), ~43 mL/min-cm2 water flow rate at 10 psi (typical results)

parameter

0.007 L/min-cm2-psi air flow rate, 140 °C max. temp., 3.36 mL/min-cm2-psi water flow rate (maximum flow rate per 1.0 psi), ~16 mL/min-cm2 water flow rate at 10 psi (typical results)

parameter

125 mL/min-cm2-psi water flow rate (maximum flow rate per 1.0 psi), 140 °C max. temp., 4.08 L/min-cm2-psi air flow rate, ~85 mL/min-cm2 water flow rate at 10 psi (typical results)

pore size

0.6 μm pore size, 5-20 % porosity

pore size

0.6 μm pore size, 5-20 % porosity

pore size

0.22 μm pore size, 13.8 % porosity

pore size

0.8 μm pore size, 5-20 % porosity

material

plain filter, white filter, polycarbonate membrane

material

plain filter, polycarbonate membrane, white filter

material

plain filter, polycarbonate membrane, white filter

material

plain filter, polycarbonate membrane, white filter

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

feature

hydrophilic

feature

hydrophilic

feature

hydrophilic

feature

hydrophilic

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    109K37981V
    028K37973
    109K37981
    109K3798
    028K37971

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