G3776

Guanosine 5′-triphosphate sodium salt solution

HPLC purified, aqueous solution for RNA polymerase transcription

• Synonym(s): GTP
• CAS Number: 36051-31-7
• MDL number: MFCD00077781
• UNSPSC Code: 41106305
• PubChem Substance ID: 24895138
• NACRES: NA.52

Properties

• biological source: Porcine brain; bacterial (Corynebacterium); yeast
• Quality Level: 300
• Assay: ≥95% (HPLC)
• form: liquid
• concentration: 100 mM in H₂O (adjusted to pH 7)
• color: colorless
• foreign activity: DNase, RNase and Nickase, none detected
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1

Description

Application

Guanosine 5′-triphosphate sodium salt solution has been used:

• for reconstituting transducin during purification of transducin.
• as a compnent of the 3.3X loading buffer to assemble 32P-miR-31—3xFLAG-AGO2 complexes
• as a component of the nucleotide substrate mix to evaluate the hydrolytic activity of MutT homolog 1 (MTH1) on methylated nucleoside triphosphates
• in DNA-dependent RNA polymerase transcription

Biochem/physiol Actions

Guanosine 5′-triphosphate (GTP) forms nearly 25% of the total nucleotide triphosphate pool and is a nucleotide anion. It participates in several biological functions such as cell signaling by activation of GTP-binding proteins, RNA and protein synthesis, and production of cyclic guanosine monophosphate (cGMP), a secondary messenger. GTP also participates in the production of stringent alarmone.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves