L1394
Lectin from Triticum vulgaris (wheat)
agarose conjugate, saline suspension
Synonym(s):
WGA, Wheat germ agglutinin
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
conjugate
agarose conjugate
Quality Level
form
saline suspension
extent of labeling
~6 mg per mL
matrix
4% agarose
matrix activation
cyanogen bromide
matrix spacer
1 atom
particle size
45-165 μm
capacity
≥0.5 mg/mL binding capacity (ovomucoid)
storage temp.
2-8°C
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Related Categories
Application
Used for the purification of glycoproteins containing (D-N-acetylglucosamine)2 or N-acetylneuraminic acid.
Biochem/physiol Actions
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Physical form
Suspension in 0.9% NaCl containing 15 mM Sodium azide.
Preparation Note
Conjugates are prepared from affinity purified lectin.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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Bo F Hansen et al.
PloS one, 7(5), e34274-e34274 (2012-05-17)
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The Journal of biological chemistry, 291(8), 3905-3917 (2015-12-20)
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D L Christie et al.
The Biochemical journal, 270(1), 57-61 (1990-08-15)
Chromaffin-granule membranes were separated into insoluble and soluble fractions after extraction with lithium di-iodosalicylate (LDIS). These fractions were characterized by one- and two-dimensional gel electrophoresis, and glycoproteins were detected after electroblotting with peroxidase-labelled concanavalin A and wheat-germ agglutinin (WGA). The
María-Ángeles Cortés-Gómez et al.
International journal of molecular sciences, 24(2) (2023-01-22)
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Protocols
This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin from GE Healthcare.
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