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M8270

Sigma-Aldrich

Anti-Mouse IgG3 (heavy chain specific) antibody produced in goat

affinity isolated antibody, lyophilized powder

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

technique(s)

Ouchterlony double diffusion: suitable

storage temp.

2-8°C

target post-translational modification

unmodified

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
IgG3 (heavy chain specific) antibody specifically recognizes heavy chain of mouse IgG3. Specificity of the mouse IgG3 is determined by immunodiffusion and IEP techniques. The purified antibody preparation is non-reactive with mouse IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM, myeloma proteins.

Immunogen

mouse IgG3 (myeloma protein)

Application

The purified IgG3 (heavy chain specific) antibody may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. IgG3 (heavy chain specific) antibody was used as secondary antibody in ELISA.

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Reconstitution

Reconstitute with 0.135 M sodium chloride.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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