MAK521
Fumarase Assay Kit
sufficient for 100 colorimetric tests
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100 μL
$703.00
$703.00
Please contact Customer Service for Availability
A recombinant, preservative-free antibody is available for your target. Try ZRB1596
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100 μL
$703.00
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
$703.00
Please contact Customer Service for Availability
A recombinant, preservative-free antibody is available for your target. Try ZRB1596
Request a Bulk Order
Recommended Products
Quality Level
detection method
colorimetric
relevant disease(s)
neurological disorders; neonatal diseases
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Fumarase (or fumarate hydratase) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate. Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc.
Features and Benefits
- Linear detection range: 0.4 to 70 U/L
- Compatible with high-throughput handling systems.
Suitability
The kit is suitable for fumarase activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)
Principle
The Fumarase Assay Kit is a non-radioactive, colorimetric assay based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
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