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RAB0178

Sigma-Aldrich

Human Fas Ligand ELISA Kit

for serum, plasma, and cell culture supernatants

Synonym(s):

FasL

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32
Pricing and availability is not currently available.
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 1.37-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FASLG(356)

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Iron rod, 1000mm, diameter 2.0mm, as drawn, 99.95%

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Iron rod, 1000mm, diameter 2.0mm, as drawn, 99.99+%

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Iron rod, 500mm, diameter 2.0mm, as drawn, 99.95%

GF86135160

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Iron rod, 500mm, diameter 2.0mm, as drawn, 99.99+%

GF47768596

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form

rod

form

rod

form

rod

form

rod

manufacturer/tradename

Goodfellow 906-251-81

manufacturer/tradename

Goodfellow 889-275-43

manufacturer/tradename

Goodfellow 861-351-60

manufacturer/tradename

Goodfellow 477-685-96

assay

≥99.95%

assay

≥99.99%

assay

≥99.95%

assay

99.99%

density

7.86 g/mL at 25 °C (lit.)

density

7.86 g/mL at 25 °C (lit.)

density

7.86 g/mL at 25 °C (lit.)

density

7.86 g/mL at 25 °C (lit.)

bp

2750 °C (lit.)

bp

2750 °C (lit.)

bp

2750 °C (lit.)

bp

2750 °C (lit.)

resistivity

9.71 μΩ-cm

resistivity

9.71 μΩ-cm

resistivity

9.71 μΩ-cm

resistivity

9.71 μΩ-cm

General description

The Human Fas Ligand ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Fas Ligand in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human Fas Ligand

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activate B cells which leads to down-regulation of the immune response.[1][2] The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to eventual cell death.[3] FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy.[4] Changes in the activity of FasL suppress normal apoptosis, leading to abnormal survival and growth of tumor cells.[5] Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome (ALPS).[6]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)
  • RABSTOP3ELISA Stop Solution (Item I)
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0502K0258
    0902I0258
    0726I0258
    1006H0258
    1007H0258

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